Capto s impact how to#

Two different MAbs are used and one of them has been run at two different conditions. When the optimized running condition was used it resulted in high resolution of monomers and aggregates (Figure 2).Ī comparison of different cation exchangers on the market is presented. Yamamoto plot for membrane chromatography (Sartobind S), B) Yamamoto plot for resin-based chromatography (Capto S ImpAct), C) Ligand binding sites for. Best aggregate removal for Capto S ImpAct at low pH and low salt concentrations. 3) 1 m diameter column and 20 cm bed height using buffers with the same viscosity as water at 20☌. To find the optimal conditions for static binding capacity of a MAb a screening was done using PreDictor™ 96-well plates. 1) Median particle size of the cumulative volume distribution 2) At room temperature using buffers with the same viscosity as water.

Capto S ImpAct is based on a newly developed grafting technology (Figure 1), resulting in high binding capacity and high resolution. The medium is designed for polishing of monoclonal antibodies (MAbs) and a wide range of other biomolecules.

Capto s impact how to#

This poster presents how to use the new cation exchange chromatography medium, Capto™ S ImpAct in MAb polishing. Capto S ImpAct Capto S ImpAct chromatography medium (resin) is a strong cation exchanger (Fig 1). A typical monoclonal antibody purification process begins with a protein A capture step, followed by one or two polishing steps often using ion exchange chromatography media.